Prolong Antifade Gold

BD Horizon Brilliant™ Violet 480 Imaging Reagents
Permeabilization buffers, as well as ProLong® Gold antifade embedding medium, which results in excellent photostability (Figure 4). The BD Horizon Brilliant™ unique polymer dyes were developed from pioneering polymer dye technology acquired from Sirigen Ltd. These dyes deliver more and brighter dye choices over ... Access Content

Protocol N-SIM Burnet - Technology Research Platforms
N"SIM&!! 2!!!! •!Coverslips!must!be!in!the!center!of!the!slide!(the!distance!fromthe!center!of!the! coverslip!to!both!short!edges!of!the!slide!must!be!the!same,!see ... Retrieve Content

Antibody + TO-PRO-3 Staining Protocol (from Molecular Probes ...
Note: The antifade properties of ProLong Gold antifade reagent improve slightly the longer it remains in contact with the specimen. To further reduce photobleaching, minimize the exposure of fluorescently labeled specimens to light by using neutral density filters, and expose samples only when observing or recording a signal. ... Retrieve Here

2-color STED CW Sample Preparation V4 June 2010
Prolong Gold (with antifade , but w/o DAPI ), a mounting which will polymerize, has been tested in STED mode and gave excellent results as long as the protein of interest is located within 10-15 µm from ... Fetch Doc

Cytoplasmic Trafficking, Endosomal Escape, And Perinuclear ...
Gold (Nanoprobes) over the capsid protein units. Free dyes or nanogold was removed from labeled viral particles by dialysis against PBS contain- (Prolong Antifade Gold with DAPI [4=,6=-diamidino-2-phenylindole]; Molecular Probes). ... Retrieve Content

HCBI Recommended Mounting Media
ProLong Gold or 97% TDE. A number of spherical aberrations were observed around bright objects (and unfortunately, enhanced with SIM processing) when mounted in ProLong Gold. Mounting in TDE resulted in an approximately 3 fold axial resolution enhancement. ... Get Content Here

FISH COFISH Protocol - Titia De Lange
FISH & COFISH Colcemid (Roche 10295892001, 10 ug/mL, stored at 4°C) Fixative ProLong Gold Antifade Reagent (Life Technologies P36934) Frosted Slides Mount using ProLong Gold, seal with nail polish, store at -20°C protected from light. SCORING . ... Get Content Here

Fitchburg, Wisconsin - Wikipedia
Fitchburg is a city in Dane County, Wisconsin, United States. The population was 25,260 at the 2010 census. The population was 25,260 at the 2010 census. Fitchburg is a suburb of Madison and is part of the Madison Metropolitan Statistical Area . ... Read Article

Surgical Sutures Filled With Adipose-Derived Stem Cells ...
ProLong Gold Antifade Reagent with DAPI (Invitrogen, Oregon, USA). Fluorescence confocal z-stacks of DAPI stained sutures were acquired with a LSM 710 microscope (Zeiss, Jena, Germany). Quantification of metabolic activity of cells in the suture At indicated time points after cell seeding, sutures were ... Access Content

User Guide: ProLong Live Antifade Reagent
ProLong™ Live Antifade Reagent Catalog nos. P36974, P36975 Introduction Loss of fluorescence signal due to photobleaching can lead to a significant loss of sensitivity for an assay. This loss in sensitivity is even more pronounced when trying ProLong 2 mL P36931™ Gold antifade reagent ... Fetch Doc

Supporting Information - PNAS
Labeled streptavidin (Invitrogen), then mounted with Prolong Antifade Gold with DAPI (Invitrogen). For macrophage stain-ing, frozen sections of freshly isolated lung specimens were in-cubated with F4/80 mAb, biotinylated anti-rat IgG, and then Alexa Fluor 488-conjugated streptavidin (Molecular Probes). Nuclei were stained with DAPI (KPL). ... Read Content

UC Davis/NIH NeuroMab Facility
UC Davis/NIH NeuroMab Facility Department of Neurobiology, Physiology and Behavior, UC Davis, Davis CA 95616-8519 www.neuromab.org neuromab@ucdavis.edu (530) 752-8398 A cooperative venture among the University of California at Davis, the National Institutes of Health, and Antibodies Inc. ... View Full Source

DAPI/Antifade Mounting Solutions - COSMO BIO
ZytoVision‘s DAPI/Antifade Mounting Solutions are ready-to-use mounting media that are applied directly to fluorescently labeled tissue or cell specimens on mi-croscope slides. They contain the nuclear counterstain DAPI (4‘, 6-diamidino-2-phe-nylindole) which produces a blue fluores-cence when bound to DNA. ... Doc Retrieval

Evaluating Crosstalk Between Epoxyeicosatrienoic Acids (EETs ...
Coverslips were mounted with Prolong Antifade Gold and imaged by conventional fluorescence microscopy (Leica). Primary Abs: rabbit anti human CYP-2J2 (1:500, a gift from Dr. Darryl Zeldin, NIEHS), rabbit anti-sEH (1:500, Cayman Chemical), goat anti-rat ... Get Document

Sample Preparation For STED CW 1 And 2-color
• Mount in Prolong Kit (or TDE + antifade). • Let cure at least overnight (at best 48 hrs to reach the maximum RI). • Keep the slide at 4 degree C and protected from the light. ... Retrieve Document

Unrivaled Protein And Cell Analysis - Fisher Scientific
Antifade mountants are designed to protect the photostability of fluorescently labeled proteins and maintain image integrity for weeks to months. Immunofluorescence analysis of mesenchymal ... Fetch Content

NeurobiologyofDisease ...
Wako). Slices were mounted with Prolong Antifade Gold with DAPI (Life Technologies). Immunofluorescence was imaged on an inverted laser scanning confocal microscope (Zeiss LSM510), and whole-slice montages were generated using a resonance-scanning confocal micro-scope (Nikon A1R). To quantify GFAP-, Iba1-, and AT8 (P-tau)-immunoreactivity, 4 20 ... Read Here

Ki67 Immunofluorescence On Bovine Cell Lines - Bio-protocol
Slides and coverslippedwith ProLong Gold Antifade Reagent with DAPI . 9. Images of immunofluorescence staining were photographed with a camera attached to a fluorescent microscope and percentage of Ki67 positive cells was calculated. 10. This staining was repeated for three independent biological replicates (Figure 1). ... Fetch Document