Promega Trypsin Gold

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Trypsin Gold, Mass Spectrometry Grade
Each lot of quality-tested Trypsin Gold, Mass Spectrometry Grade, is qualified for use with in-gel digestion and mass spectrometric analysis. A copy of the spectrogram generated with carbonic anhydrase II and any lot of Trypsin Gold, Mass Spectrometry Grade, may be obtained from Promega Technical Services at 1-800-356-9526. ... Get Document

Trypsin Gold, Mass Spectrometry Grade ... - Promega Corporation
E-mail Promega Technical Services if you have questions on use of this system: techserv@promega.com Trypsin Gold, Mass Spectrometry Grade 1. Description Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues. The distribution ... Read Content

Trypsin NB Premium Grade, MS Approved Trypsin NB ... - SERVA
And Promega Trypsin Gold, Mass Spectrometry Grade in reversed phase HPLC. SERVA Trypsins are highly purified enzyme preparation that are free of activity from other proteases. The absence of chymotryptic activity is verified by purity and function control which ... Access Document

In-gel Trypsin Digestion - Université De Sherbrooke
We use Trypsin Gold from Promega (V5280), which is supplied as 100 g of powder. We resuspend it at 1 g/ l as our stock solution (i.e. add 100 l of 50mM acetic acid (see note) to the 100 g powder in the vial, and store it at –80oC in 10 l aliquots). Trypsin is not stable especially when pure and in non- ... Return Doc

Trypsin In-solution Digestion - Howard University
Trypsin In-solution Digestion Your protein is in a volume ~20 ul (~ 100 nmole/ ~ 4 ug total protein) Add 2 ul 100 mM DTT (final 10 mM), incubate 30 min hour at 60 C, Add 2 ul iodoacetamide (0.15 M in 200 mM Ammonium Bicarbonate). Incubate 20 min at room temperature in the dark ... Retrieve Here

Get Published In Science, Meet
©2008 Promega Corporation,16134-AD-GN Now get enhanced digestion of even the most difficult proteins and more complete peptide recovery from gels.The combination of Promega Trypsin Gold and ProteasMAX™ Surfactant gives you maximum protein sequence coverage and increased confidence of correct protein identification. ... Access Full Source

Tools To Improve Protein Sample Preparation For Mass Spec
Promega Corporation Trypsin, Sequencing Grade Highest digestion efficiency Tolerance to protease inhibiting agents Digestion efficiency Higher digestion efficiency Higher purity Overall good performance Enhancing trypsin performance Trypsin Gold, Mass Spec Grade Trypsin/Lys-C mix ... View This Document

In-Gel Digestion Using ProteaseMAX 040412
Trypsin stock solution 0.1 µg/µl for low abundance protein Add 1 ml of HPLC H2O to 100 µg trypsin lyophilized powder (Trypsin Gold Cat.# V5280 from Promega) Aliquot into 2-5 µl Snap-freezing on dry ice or nitrogen prior to storage at -70oC Reagents required 1 M Ammonium bicarbonate (AmBic or NH4HCO3) ... Read More

Sequencing Grade Modified Trypsin ... - Promega Corporation
Promega Sequencing Grade Modified Trypsin is porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion (2). In enzymatic stability tests, modified trypsin was found to retain greater than two times the Trypsin Gold, Mass Spectrometry Grade 100µg ... Access Content

PSR O18 Labeling Procedure - Ohsu.edu
PSR 18 O Labeling Procedure adapted from Larry’s Lab 07/07/2007 Reagents Trypsin Gold (Promega Cat # V5280) DiH 2O Neat Formic Acid (FA) Poroszyme Immobilized Trypsin beads (Applied Biosystems Cat # 2-3127-00) ... Read Full Source

Www.etsu.edu
Following In-gel tryptic digestion kit by Pierce.. Material prep. Trypsin. stock - Promega Trypsin gold MS grace porcine 100 µg Cat#V528A, hydrated with trypsin storage solution or reconstitute in 50 mM acetic acid. ... Content Retrieval

In-Gel Tryptic Digestion Protocol - UAB
O Modified Trypsin: (Promega cat #V528A; Trypsin Gold Mass Spectrometry Grade; 100µg/vial) Carefully dissolve one pellet in the vial with 1000µL of 50 mM ammonium bicarbonate to make a working 0.1mg/mL stock solution. Aliquot into 0.5mL tubes, store in -20°C. Limit freeze-thaw cycles to 5. ... Read More

Characterization Of A Grape Class IV Chitinase - Pubs.acs.org
In-solution trypsin (Promega Trypsin Gold) digestion in the presence of a protease enhancer (Promega ProteaseMAX Surfactant) according to the manufacturer’s protocol. LC-MS/MS analysis was conducted on an LTQ-FT MS (Thermo Fisher Scientific, San Jose, CA, USA) ... Access Document

In-Solution Digestion Protocol Using TCEP & IAA
1 ug/ul Trypsin solution 5 ul aliquots of 1 ug/ul Trypsin in 0.5 ml microtubes are stored in the -80C. [To make the 1 ug/ul stock, mix 100 ul HPLC water with 100 ug of Trypsin Gold from Promega (V5280).] Digestion Procedure 1) Reconstitute dried protein sample in 40 ul of 8.0 M urea in 100 mM Tris-HCl (pH 8.5) ... Fetch Document

Partial trypsin Digestion Of Cornified Envelope Proteins ...
• trypsin (We recommend Trypsin Gold, Mass Spectrometry Grade, Promega Cat.# V5280.) • 50mM acetic acid The following reagents are also required for in-solution digestions. • –20°C acetone • 8M urea The following conditions are for a pilot experiment in which trypsin concentration and digestion time are varied. ... Document Viewer

Dried Blood Spot Proteomics: Surface Extraction Of Endogenous ...
Preparation via trypsin digestion by use of the Advion Biosciences Triversa Nanomate robotic platform. Liquid chromatography tandem mass spectrometry of (Trypsin Gold; Promega, Southampton, UK). The three DBS were sampled and digested in parallel to demonstrate the capability ... Fetch This Document

Purpose - National Cancer Institute
Trypsin Gold (Promega): 100μg resuspended in 1mL of 0.2M Tris pH 8. (see above). Quench: 4 parts HPLC water. 1 part formic acid. Procedure. Preparation of Samples. Dilute the cell lysate to 2mg/mL with lysis buffer. Move 50uL of cell lysate to a deep well plate. ... Access This Document

Peptide Sequences From The First Castoroides Ohioensis Skull ...
Iodoacetamide for 1 h in the dark, then digested with Promega Trypsin Gold (1:100, trypsin: bone protein) overnight at 378C. The resultant peptides were desalted using Empore C18 (3 M) stage tips [22]. roads bodies of water topographic contour lines kame deposits lacustrine beach lacustrine silt and clay outwash sand and gravel swamp deposits ... Retrieve Doc

Www.etsu.edu
Promega’s Trypsin Gold In Solution Digest Protocol9/28/12 MFB Wear nitrile gloves and make sure area is free of dust and lint by wiping down area with ethanol using lint-free wipes. Add 11.4 μl of 6 M urea, 100 mM Tris-HCl, pH 8.5 to every 2 μl of protein. ... Get Doc

Certificate Of Analysis Sequencing Grade Modified Trypsin ...
Specificity (1). Promega Sequencing Grade Modified Trypsin is porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion (2). In enzymatic stability tests, modified trypsin was found to retain greater than two times the activity of unmodified trypsin. ... Retrieve Here